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Sykam – The world's leading Amino Acid Analyzer (AAA) manufacturer

Sykam – The world's leading Amino Acid Analyzer (AAA) manufacturer

Sykam GmbH is a German company that, thanks to its deep expertise in the manufacturing and development of chromatography equipment and compliance with international standards such as ISO 9001 and 13485, has been one of the world's leading Amino Acid Analyzer (AAA) suppliers since 1980. In addition, Sykam's chromatography portfolio includes Ion Chromatography, Preparative Chromatography, and HPLC.

The automated amino acid analyzer is based on the principle of separating free amino acids via cation-exchange chromatography, followed by post-column derivatization with Ninhydrin and detection at 440 and 570nm; this is a mandatory method compliant with European Commission Directive 98/64/EC.

Sykam – The world's leading Amino Acid Analyzer (AAA) manufacturer

Sykam AAA can be used for a wide range of applications:

  • Animal feed
  • Food
  • Organic amines
  • Pharmaceutical quality control
  • Clinical specimens

Sykam AAA offers numerous advantages:

  • High resolution and fast analysis
  • High sensitivity combined with a wide dynamic range
  • Stable baseline with minimal noise – even for complex matrices because amino acids are separated from the sample matrix prior to derivatization

Control via Internet/LAN, RS232, and/or USB

Clarity chromatography software is a chromatography data acquisition and reporting software capable of controlling up to 4 chromatography systems from 1 PC in compliance with current GLP guidelines and FDA CFR Part 11 regulations.

Sykam – The world's leading Amino Acid Analyzer (AAA) manufacturer - Fig. 2

Sykam AAA consists of 4 main parts

  1. PUMP SYSTEM
    Low-pressure mobile phase gradient pump with maximum flow rate 10L/min, maximum pressure 350 bar
    Reagent pump with maximum flow rate 2L/min, maximum pressure 350 bar
    Online Degasser up to 7 channels, <20% dissolved gas
  2. AUTOSAMPLER SYSTEM
    Dual syringe pump to prevent system clogging

Sykam – The world's leading Amino Acid Analyzer (AAA) manufacturer - Fig. 3

Optional high-temperature reaction chamber for running easily degradable samples, combined with a fluorescence detector

  1. REACTION MODULE
    Column oven +30°C – 99°C
    Reaction chamber 30°C – 150°C ensuring fast and stable ninhydrin reaction
    2-Channel LED Detector 440 & 570 nm
  2. REAGENT STORAGE unit for ninhydrin ~ 15°C to maintain stability

Sykam – The world's leading Amino Acid Analyzer (AAA) manufacturer - Fig. 4

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